Stable compositions of dehydroascorbic acid

ABSTRACT

The invention relates to stable liquid compositions containing the oxidized form of vitamin C known as dehydroascorbic acid (DHAA). The compositions comprise DHAA and a pharmacologically acceptable liquid organic polyol solvent. The polyol solvent comprises about 50% or greater of the total weight of the composition. The compositions are useful as dietary supplements, skin-enhancers, concentrates, or research solutions.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation-in-part of application Ser. No. 12/787,331, filed May 25, 2010. The patent application identified above is incorporated here by reference in its entirety to provide continuity of disclosure.

BACKGROUND

1. Field of Invention

This invention relates to compositions of matter used as sources of vitamin C in dietary supplementation, skin care products, therapy, research, and manufacturing. More specifically, the invention relates to stable liquid compositions containing the oxidized form of vitamin C known as dehydroascorbic acid.

2. Prior Art

Ever since the elucidation of the chemical structure of vitamin C in the mid-1930's it has been known that vitamin C occurs naturally as two different compounds, namely, ascorbic acid (AA) and an oxidized form of AA called dehydroascorbic acid (DHAA). It also is known that AA and DHAA are unstable compounds. In aqueous solutions, some factors which affect the rate of their destruction include the pH of the solution, and exposure to various metal ions, heat, light and air. It also is known that DHAA is considerably less stable than AA when subjected to comparable conditions. ‘Deutsch J C. Dehydroascorbic acid. Review Journal of Chromatography A, 881 (2000) 299-307’ (Deutsch), incorporated here by reference, states en-equivocally “DHA is more reactive and unstable in solution than AA.” Therefore, as a supplement to the diet, or as an ingredient of a topically applied product such as a skin lotion, AA has been the preferred chemical form of vitamin C because of its greater stability. In fact, we do not know of any commercially available dietary or topically applied product wherein DHAA specifically has been utilized as a substantial source of vitamin C.

Also known is that solid AA is far more easily dissolved in water than is solid DHAA, as noted in ‘Pecherer B J. The Preparation of Dehydro-L-ascorbic Acid and its Methanol Complex. Am Chem Soc 73 (1951) 3827-3830’ (Pecherer) and ‘Koliou E K and Ioannou P V. Preparation of dehydro-L-ascorbic acid dimer by air oxidation of L-ascorbic acid in the presence of catalytic amounts of copper(II) acetate and pyridine. Carbohydrate Research 340 (2005) 315-318’ (Koliou) which are incorporated here by reference. To prepare aqueous solutions of DHAA from the solid form requires prolonged mixing at temperatures well above 37 degrees centigrade. Thus solutions of DHAA are much more difficult to manufacture than solutions of AA. Also, since the conditions to solubilize it efficiently do not exist in the gut of human or other animals, substantial doubt exists about whether the dry, solid form of DHAA can be absorbed when ingested. These are also reasons why DHAA has not been utilized as the source of vitamin C for dietary supplements or topical products.

Around the same time as the chemical structures of AA and DHAA were elucidated in the mid-1930's, the antiscorbutic properties (ability to prevent the disease called scurvy) of both compounds were recognized and generally accepted as being equal or nearly so. The oxidation of AA to DHAA was shown to be reversible both in vitro and in biological systems, so the equivalence of the two compounds could easily be attributed to simple interconversion within an organism. Although a few early investigators did note some peculiar differences in the biological utilization of these two compounds, at least as essential dietary ingredients for humans and certain other species, AA and DHAA were generally considered bioequivalent. The dietary supplement and skin care product industries developed their products using AA (and various more stable derivatives of AA) because of the stability and solubility issues with DHAA, and DHAA has essentially been ignored and forgotten in these industries.

Since the mid-1930's, the volume of research in vitamin C has been enormous, and it is possible that no single subject in the field of biology has been the focus of more research and more scientific journal articles than vitamin C. And since about the mid-1990's, many new discoveries about DHAA have been made. Among these discoveries, those of particular pertinence to the present invention include those which demonstrate that, although the two compounds are equivalent in their antiscorbutic properties, AA and DHAA are not “bioequivalent” in any broad definition of the word. Specifically it is known today that AA and DHAA are absorbed by different mechanisms in the gut; that they accumulate differently in the various tissues of an animal when ingested; that they are absorbed into living cells by completely different mechanisms utilizing different receptors on the cell surface; that the cells of certain important tissues of the human body (e.g., brain) have a very high concentration of vitamin C but completely lack cell surface receptors for AA; that DHAA is absorbed into cells by the same receptors as glucose, which are present on every cell in the human body; that in human skin cells, DHAA is absorbed up to 5 times faster and to levels 2 times higher than is AA; that DHAA is almost instantly converted into AA once it has been absorbed into a cell; that both AA and DHAA have antiviral effects in vitro against viruses that cause disease in humans such as HSV-1 (herpes simplex virus type 1 that causes oral herpes and can cause genital herpes), influenza virus, and poliovirus; and that DHAA has much stronger antiviral effects than does AA. Literature that supports these statements, and is incorporated here by reference, includes ‘Savini et al. Dehydroascorbic acid uptake in a human keratinocyte cell line (HaCaT) is glutathione-independent. Biochem J 345 (2000) 665-672’ (Savini) and ‘Furuya et al. Antiviral effects of ascorbic and dehydroascorbic acids in vitro. Int J Mol Med 22 (2008) 541-545’ (Furuya).

Thus it can be seen that a solution of DHAA for oral ingestion or topical application, while being a source of vitamin C much like numerous other available products that contain AA, also can provide specific benefits and uses unavailable in any other product on the market today. What is needed is a stable liquid solution of DHAA in an orally and topically acceptable medium.

U.S. Pat. No. 5,140,043 (Darr) discloses topical compositions of ascorbic acid (or a reducing analog of ascorbic acid) in a water-(glycol or polyol) carrier, wherein the ratio of water to glycol/polyol carrier is high (e.g., at least 1:1). These solutions of Darr do not contain DHAA, and Darr is silent as to the stability of the non-reducing compound DHAA in this carrier. We have found that DHAA is not stable in polyol solutions containing such high concentrations of water, which points out that no assumptions about the chemical and physical behavior of DHAA in polyol solutions should be drawn from the behavior of AA in those solutions. While AA and DHAA share certain biological functions, they are two different molecules in regard to their physical and chemical behavior, including stability.

U.S. Pat. No. 6,197,813 (Hegenauer) discloses stable vitamin C compositions of mineral ascorbates in liquid organic polyol solvents having pH values of about 5 to 7, but is silent as to the stability of the non-mineral DHAA in those solvents. These compositions of Hegenaur do not contain DHAA. In fact, these compositions do not even contain a naturally-occurring form of vitamin C, and therefore if these compositions were applied to the skin, vitamin C would not be expected to be absorbed by either the ascorbic acid receptors or the glucose receptors of skin cells.

US Patent Application 2009/0016974 A1 (Pruche et al) discloses DHAA-containing compositions formed “in situ” from ascorbic acid via chemical oxidation and/or via enzymatic oxidation, and a two-component agent thereof. These compositions attempt to overcome the instability of DHAA by preparing it fresh as needed, but they require handling and mixing steps of the two-component agent. The two components must be stored separately. Chemical oxidizers are harsh and can be dangerous, and enzymes are unstable, thus these compositions are problematic in regard to safety and reliability. Since the two components are intended to be combined by the end user, the temperature of the reaction and other conditions necessary for reliable oxidation processes are beyond the control of the manufacturer. Without some separate indicator, the final consumer cannot be assured that the solution prepared by the two-component system actually contains DHAA, because the oxidation of AA to DHAA is not visually or otherwise simply detected. These compositions do not contain DHAA pre-prepared in a stable solution, and Pruche et al is silent as to the stability of DHAA in the disclosed compositions.

Objects and Advantages

Several objects and advantages of the present invention are:

-   -   a. To provide compositions containing DHAA in a stable form.     -   b. To provide stable DHAA-containing compositions for topical         application to the skin of a human or animal as a source of         highly absorbable vitamin C.     -   c. To provide compositions for topical application that are         pharmacologically acceptable and pleasant to use.     -   d. To provide compositions for topical application that can be         applied alone, or mixed with water to provide greater         humidifying effect, or mixed with another skin care product to         enhance the vitamin C content of that product.     -   e. To provide compositions containing DHAA for topical         application that can also solubilize other skin-enhancing         substances that are insoluble in water, such as vitamin E.     -   f. To provide stable DHAA-containing compositions for dietary         supplementation of a human or animal as a source of highly         absorbable vitamin C.     -   g. To provide compositions for dietary supplementation that are         pharmacologically acceptable and pleasant to use.     -   h. To provide compositions for dietary supplementation that can         be taken orally alone, or mixed with water or some other liquid,         or applied to solid food.     -   i. To provide stable DHAA-containing concentrates for         manufacturing of other products.     -   j. To provide stable DHAA-containing compositions that can be         conveniently used in research, for example in chemical studies,         or in microbial culture or tissue culture.     -   k. To provide stable DHAA-containing compositions that do not         require the addition of chemical stabilizers or preservatives.

DRAWING FIGURES

FIGS. 1 to 8 show the DHAA stability of the various compositions described in Example 1 as compared with DHAA prepared similarly in water.

FIG. 9 shows the DHAA stability of the composition described in Example 2 as compared with DHAA prepared in water.

FIGS. 10 to 15 show the DHAA stability of the compositions described in Example 3 as compared with DHAA prepared similarly in water.

FIGS. 16 to 20 show the DHAA stability of the solutions described in Example 4.

FIG. 21 shows the absorption of AA and DHAA into skin as described in Example 5.

DESCRIPTION

We have discovered that DHAA is stable in solutions of pure polyol solvents and in solutions wherein the polyol content is greater than about 50 percent. By “stable” is meant that DHAA in these solutions deteriorates very slowly over a sufficient period of time that it can be stored and sold as a dietary supplement or as a skin care product, or as a concentrate for preparing or manufacturing them, with a reasonable shelf life.

In one embodiment, a DHAA composition is provided wherein the composition comprises 50% or more polyol by weight and a ratio of DHAA to AA of from greater than 1:100, 1:50, 1:10, 1:5, 3:10, 1:2, 1:1, 2:1, 10:3, 5:1, 10:1, 50:1, or 100:1.

In one embodiment, a DHAA composition is provided wherein the composition comprises 50% or more polyol by weight and greater than 0.05%, 0.25%, 0.5%, 0.83%, 1.7, or 2.5% DHAA.

In one embodiment, a DHAA composition is provided wherein the composition comprises 50% or more polyol by weight and about 0.85% to 15% DHAA.

In one embodiment, a method of treating a disease or condition is provided comprising identifying an individual in need of treatment and administering to said individual a DHAA composition comprising 50% or more by weight of a polyol. In one aspect, the DHAA composition comprises a pharmaceutically acceptable carrier. In one aspect, the composition is suitable for oral administration. In one aspect, the composition is suitable for intravenous administration. In one aspect, the composition is suitable for intraperitoneal administration. In one aspect, the composition is suitable for topical administration.

In some embodiments, the method of treating a disease or condition comprises identifying an individual having a disease or condition that may be treated by DHAA, AA, or a combination thereof.

In some embodiments, the method of treating a disease or condition further comprises administration of a second agent. In some aspects, the second agent is an anti-cancer agent, an antiviral agent, or an agent used for treating a neurodegenerative disease or condition.

The solutions are made by oxidizing ascorbic acid that is first dissolved in a pure polyol solvent, or in water, or in some mixture of these liquids. The polyol concentration may be adjusted to about 50% or greater prior to oxidizing the AA or afterwards.

The solutions can also be made by oxidizing AA that is dissolved in an alcohol (e.g., ethanol), and then combining the DHAA-containing alcohol with a polyol solvent. If it is desired that the final solution does not contain alcohol, the alcohol can be removed by evaporating the alcohol from the polyol solvent solution using heat or vacuum, or both.

The solutions can also be made by dissolving solid DHAA in a pure polyol solvent, or in water, or in some mixture of these liquids. The polyol concentration may be adjusted to about 50% or greater prior to dissolving the DHAA or afterwards.

The organic polyol solvents are chosen for pharmaceutical and dietary acceptability, their ability to solubilize the AA and DHAA component, water content, and effect on the stability of the DHAA component. At present we prefer to employ commercially available glycerol which generally contains 5% or less water. In general, we prefer to minimize the water content of the solvent(s), consistent with economic and functional considerations. Other polyols which can be employed include propylene glycol, hexylene glycol, butylene glycol and the almost infinite molecular weight range of polyethylene glycols, as well as so-called sugar alcohols, e.g., sorbitol and xylitol, and mixtures thereof with other polyols.

These solutions can be prepared entirely with one polyol solvent, e.g., glycerol, or mixtures of polyol solvents. The final choice of solvent will depend on economics and other relevant factors.

Methods we have successfully applied for oxidizing the ascorbic acid include the use of halogen or ozone or oxygen/activated charcoal or Fenton's Reagent or ascorbic acid oxidase enzyme. All of these methods are known in the art, as are other methods; the previously cited references Pecherer and Koliou show typical applications of various methods for example. The method by which the oxidation is accomplished is not the determinant factor of the long term stability of the DHAA in the solution, and other methods of oxidation are within the scope of the invention.

AA concentration in solution is commonly measured as the reducing activity of the solution using starch-iodine titration methods that are well-known in the art. AA is also measured by ultra-violet spectrophotometry using a wavelength at which AA absorbs strongly and DHAA does not, typically about 265 nm. This method is also well known in the art. DHAA in solution can be converted into AA by reducing agents such as dithiothreitol (DTT) or tris(2-carboxyethyl)phosphine (TCEP), and its concentration is commonly measured spectrophotometrically as the difference in absorbance of a solution subjected to reduction by DTT or TCEP versus a similar solution that is not subjected to a reducing agent. These methods are also well-known in the art, but see Deutsch for examples. In the description, claims, and the following examples, DHAA in the compositions of the invention is the vitamin C that can be measured by the difference in absorbance at 262 nm using TCEP reducing agent.

The following embodiments are exemplary of the invention:

Example 1

In a preferred embodiment of the invention, AA dissolved in glycerol and/or water is oxidized using ozone to produce DHAA solutions. Water-based solutions and glycerol-based solutions may be combined to yield stable DHAA compositions having the desired polyol concentration.

A 15% AA solution in water was prepared by adding 15 grams AA per 100 mL purified water with stirring. A 15% solution of AA in glycerol was prepared by adding 15 grams AA per 100 mL pure USP glycerol and stirring with heat. A corona-discharge type ozone generator with feed-gas of pure oxygen was used to supply an oxidizing gas containing about 5% ozone, and each of the 15% AA solutions was subjected to oxidizing conditions by bubbling the oxidizing gas through the solution using a glass diffuser. The progress of AA oxidation in each solution was monitored by the disappearance of reducing activity as measured by starch-iodine titration. Each solution was subjected to the oxidizing conditions until all (>99%) of the original reducing activity had disappeared. The solution made with pure glycerol was labeled “100% Glycerol,” and the solution made with purified water was labeled “100% Water.” Portions of these two solutions were combined to produce solutions of various glycerol concentrations by weight, specifically “99% Glycerol,” “98% Glycerol,” “97% Glycerol,” “96% Glycerol,” “95% Glycerol”, “90% Glycerol,” and “50% Glycerol.” For example, 99 parts by weight of “100% Glycerol” was combined with 1 part by weight “100% Water” to produce the “99% Glycerol” solution.

Aliquots of each of the solutions prepared above were placed in translucent, screw-capped polyethylene vials and were stored at room temperature. No attempt was made to further protect the vials from ambient indoor light, and each vial contained a headspace of normal air. Each vial was periodically opened to remove a sample for stability testing over the next 229 days. The concentration of DHAA in each sample was measured by spectrometry on each testing day. The initial DHAA concentration of each solution on Day 1 was recorded and assigned a value of 100%, and the concentration on each subsequent stability test day was calculated as the percent remaining of the initial concentration.

FIGS. 1 through 8 show the results of stability testing of the various glycerol-containing solutions; each graph also shows the result of the “100% Water” solution for comparison. It can be seen that DHAA decomposes rapidly in water. By the time the water solution was tested at 20 days, less than 10 percent of the initial amount of DHAA remained. By contrast, DHAA is preserved very well in solutions containing high concentrations of glycerol. In pure glycerol for example, greater than 80% of the initial DHAA concentration remains even after approximately 8 months of storage at room temperature. As the glycerol concentration is reduced, stability is reduced, until only minor improvement is gained at 50% glycerol concentration.

Example 2

In another embodiment, a stable DHAA composition is produced by oxidation of AA dissolved in glycerol using exposure to activated charcoal and oxygen as the oxidation method.

A solution of AA in pure USP glycerol was subjected to oxidizing conditions by suspending activated charcoal in the solution and then bubbling pure oxygen through the solution. Oxidation of AA during this process was monitored by starch-iodine titration. After the desired amount of AA had been oxidized, the activated charcoal was removed from the solution by centrifugation and filtration. This solution was labeled “100% Glycerol.” A portion of the solution was then placed in a translucent, screw-capped polyethylene vial and was stored at room temperature. No attempt was made to further protect the vial from ambient indoor light, and the vial contained a headspace of normal air. The vial was periodically opened to remove a sample for stability testing over the next 191 days. The concentration of DHAA in the sample was measured by spectrometry on each testing day. The initial DHAA concentration of the solution on Day 1 was recorded and assigned a value of 100%, and the concentration on each subsequent stability test day was calculated as the percent remaining of the initial concentration.

FIG. 9 shows the results of the stability testing of this solution, and for comparison also shows the stability of a DHAA solution prepared in purified water (labeled “100% Water”). It can be seen that DHAA in glycerol produced by an alternative oxidation method shows excellent long-term stability.

Example 3

In another embodiment, stable DHAA compositions are produced by oxidizing AA dissolved in water using Fenton's Reagent as the oxidizing method, and then combining the water solution with propylene glycol such that the final concentration of polyol is 50% or greater.

AA was dissolved in purified water to give a highly concentrated solution, and then sufficient 30% hydrogen peroxide was added to oxidize about half of the AA. Iron to catalyze the reaction was provided by addition of ferrous sulfate. Oxidation of the AA was monitored by spectrometry until the expected amount of AA had been oxidized. This solution was labeled “100% Water.” Portions of this solution were combined with portions of pure, USP grade propylene glycol to produce solutions of “97% Propylene Glycol,” “95% Propylene Glycol,” “90% Propylene Glycol,” “80% Propylene Glycol,” “70% Propylene Glycol,” and “50% Propylene Glycol.” For example, 3 parts by volume of the “100% Water” solution were combined with 97 parts by volume propylene glycol to yield the “97% Propylene Glycol” solution.

Aliquots of each of the solutions prepared above were placed in translucent, screw-capped polyethylene vials and were stored at room temperature. No attempt was made to further protect the vials from ambient indoor light, and each vial contained a headspace of normal air. Each vial was periodically opened to remove a sample for stability testing over the next 31 days. The concentration of DHAA in each sample was measured by spectrometry on each testing day. The initial DHAA concentration of each solution was recorded and assigned a value of 100% (Day 0), and the concentration on each subsequent stability test day was calculated as the percent remaining of the initial concentration.

FIGS. 10 through 15 show the results of stability testing of the various propylene glycol-containing solutions; each graph also shows the result of the “100% Water” solution for comparison. It can be seen that DHAA decomposes rapidly in water; after only 5 days, less than 20 percent of the initial amount of DHAA remains. By contrast, DHAA is preserved very well in solutions containing high concentrations of propylene glycol. In fact, the DHAA concentration in many of these solutions actually increased significantly over time, a remarkable and unexpected discovery. We believe this phenomenon can be explained this way: residual AA continues to oxidize while the DHAA is stabilized and therefore accumulates in the solution. The spectrophotometric measurements support this explanation, but we do not wish to be bound by this explanation.

Example 3

demonstrates that stable DHAA compositions may be prepared using a third alternative oxidation method as compared with the first two examples, and also demonstrates that an alternative polyol solvent can be used.

Example 4

In another embodiment, stable DHAA compositions containing various ratios of AA:DHAA are demonstrated.

A first solution was prepared containing AA and DHAA in 100% glycerin, wherein the ratio of AA:DHAA was about 1:1, and the actual concentrations of AA and DHAA was about 2.5% by volume respectively. Therefore the total vitamin C concentration in the first solution was about 5%. A second solution was prepared containing only AA in 100% glycerin, and wherein the AA concentration was about 5% by volume. Various proportions of the first solution and the second solution were combined to produce other solutions that each contained a total vitamin C concentration of about 5%, but wherein the ratio of AA:DHAA varied between about 1:1 and about 99:1. More specifically, solutions were prepared that contained AA:DHAA ratios of about 2:1, 5:1, 9:1, 19:1, and 99:1.

For comparison, a solution was prepared containing AA and DHAA in 100% water, wherein the ratio of AA:DHAA was about 1:1, and the total vitamin C content was about 5%. This solution was designated “Control.”

Aliquots of each of the solutions prepared above were placed in transparent, screw-capped glass vials and were stored at room temperature. No attempt was made to further protect the vials from ambient indoor light, and each vial contained a headspace of normal air. Each vial was periodically opened to remove a sample for stability testing over the next 30 days. The concentration of DHAA in each sample was measured by spectrometry on each testing day. The initial DHAA concentration of each solution was recorded and assigned a value of 100% (Day 0), and the concentration on each subsequent stability test day was calculated as the percent remaining of the initial concentration.

FIGS. 16-20 show the results of stability testing of the various solutions; each graph also shows the result of the “Control” solution for comparison. It can be seen that DHAA decomposes rapidly in water when the initial ratio of AA:DHAA is about 1:1; after only 3 days, less than 20 percent of the initial amount of DHAA remains. By contrast, DHAA is preserved very well in polyol solutions containing AA:DHAA ratios of between 2:1 and 99:1

Example 5

A solution containing both AA and DHAA dissolved in pure glycerol was prepared. Concentrations of AA and DHAA were about 2.5% respectively. This solution was applied topically to the skin of human volunteers to compare the absorption rate of AA versus DHAA. To assess permeation into the stratum corneum, the solution was diluted 1:1 with water and applied at a rate of 1.4 mg/cm² to delineated skin surfaces and allowed 0, 2 or 4 hours for absorption. Each skin surface was then washed with deionized water, and the skin washings were collected and measured for AA and DHAA content. The amount absorbed at 2 and 4 hours was determined by subtraction from the amount present in the 0 hour washings.

FIG. 21 shows the absorption of AA and DHAA into skin. Data points are the mean+/−SD of triplicate analyses on 4 subjects. The 2 and 4 hour values are the difference as percentage from the 0 hour (baseline) values, determined by analysis of skin washings. It can be seem that after 4 hours DHAA had absorbed into the skin at a rate about 12 times greater than AA, and that the total vitamin C content of the skin was increased at a much more rapid rate than applying AA alone. (Asterisks on FIG. 21 indicate the following: * Statistically significant at P<0.05. ** Statistically significant at P<0.01).

Example 6

A solution containing both AA and DHAA dissolved in pure glycerol was prepared. Concentrations of AA and DHAA were about 2.5% respectively. One part solution was diluted with 9 parts water to give a working solution. The AA and DHAA concentrations of this working solution were measured and recorded. About 20 grams of this solution was taken into the mouth by a human volunteer. Over the course of 5 minutes, the volunteer intermittently rinsed the oral cavity with the working solution by swiahing the solution about in the mouth, or gargled with the solution to rinse the tissues of the throat, being careful to avoid swallowing any of the working solution. At the end of 5 minutes, the volunteer expectorated the fluid, which was collected and weighed. The AA and DHAA concentrations of the expectorated fluid were measured and recorded. The amount of AA and DHAA absorbed into the tissues of the mouth and throat was determined by subtraction from the concentrations determined on the solution before application to the mouth. Results were corrected for the dilution effect of accumulated saliva according to the difference in weight of fluid taken into the mouth as compared to the weight of the expectorated fluid. The results showed that approximately 13.2% of the total AA was absorbed, and that approximately 14.8% of the DHAA was absorbed.

CONCLUSION, RAMIFICATIONS, AND SCOPE

While the above description contains many specificities, these should not be construed as limitations on the scope of the invention, but rather as exemplification of preferred embodiments. The compositions can be prepared using various methods and ingredients as mentioned, and their equivalents. Polyol solvents are known to be antimicrobial in high concentrations and therefore the compositions of the invention generally do not require preservatives. Polyol solvents are also capable of dissolving substances that are not soluble in water, so are capable of solubilizing not only AA and DHAA but additional dietary or skin-enhancing ingredients such as vitamin E. Many polyol solvents are excellent skin-enhancing substances in their own right, such as glycerol which is commonly utilized in skin care products as a humectant. Many polyol solvents are not only safe for ingestion, but in fact have a pleasant, sweet flavor. Thus the compositions have favorable properties that are synergistic with their use as dietary supplements, skin-enhancers, concentrates, or research solutions. Accordingly, the scope of the invention should be determined not by the embodiments illustrated, but by the appended claims and their equivalents. 

We claim:
 1. A liquid vitamin C composition, comprising: (a) at least dehydroascorbic acid (DHAA), and (b) at least one pharmacologically acceptable liquid organic polyol solvent for said DHAA; wherein said polyol solvent comprises about 50% or greater of the total weight of said composition, further comprising a ratio of DHAA to ascorbic acid (AA) is greater than 1:9.
 2. The liquid concentrate composition of claim 1 wherein said composition includes a pharmacologically acceptable vitamin E compound.
 3. A liquid dehydroascorbic acid (DHAA) composition, comprising: (a) at least DHAA, and (b) at least one pharmacologically acceptable liquid organic polyol solvent for said DHAA; wherein said polyol solvent comprises about 50% or greater of the total weight of said composition, wherein the amount of DHAA is greater than 0.1%.
 4. The liquid vitamin C composition of claim 1, wherein said ratio of DHAA to AA is greater than 1:5.
 5. The liquid vitamin C composition of claim 1, wherein said ratio of DHAA to AA is greater than 1:2.
 6. The liquid vitamin C composition of claim 1, wherein said ratio of DHAA to AA is greater than 1:1.
 7. The DHAA composition of claim 3 wherein DHAA comprises about 0.75% to about 15% of said composition.
 8. The DHAA composition of claim 3 prepared by oxidizing ascorbic acid in solution by exposing said solution to halogen or ozone or oxygen/activated charcoal or Fenton's Reagent or ascorbic acid oxidase enzyme or a gas containing oxygen and/or ozone wherein said gas contains said oxygen and/or ozone at concentrations greater than found in typical atmospheric air.
 9. The DHAA composition of claim 3 prepared by oxidizing ascorbic acid and combining with a substantially pure polyol.
 10. The DHAA composition of claim 3 which is a dietary supplement and/or for topical application.
 11. The DHAA composition of claim 3 comprising a dietary acceptable polyol.
 12. The DHAA composition of claim 3 comprising a topically acceptable polyol.
 13. The DHAA composition of claim 3 in a polyethylene or glass container.
 14. The DHAA composition of claim 3 wherein said DHAA is stable at room temperature.
 15. The DHAA composition of claim 3 wherein the level of DHAA is stable or, increases. 